Effect of oFSH dosages in superovulation protocolon ovarian responses in goat
نویسنده
چکیده
A total of 35 crossbred goats were used to evaluate the effect of oFSH dosages on ovarian responses during superovulation. CIDR was inserted for 10-15 days and were divided into 3 groups, namely Groups M,, M2 and S receiving 8.8 mg and 14.08 mg of oFSH through multiple intramuscular (i.m.) injection starting from 2 days before the CIDR removal and 8.8 mg of oFSH through single i.m. injection upon CIDR removal, respectively. Ovarian responses of all treatments were evaluated during laparotomy session on days 3 or 7 after CIDR removal. All the does (100%, 22/22) of Group Ml and M2 responded to the treatments by ovulating at least 1 corpus luteum (CL). On the other hand, only 69% (9/13) of the does of Group S was responded correspodingly to the treatment. The total ovarian responses (CL plus anovulatory follicles) among the treatments were significantly (P<0.05) higher in multiple administration groups (Groups Ml and M2) than the single administration group (Group S). Although no significant (P>0.05) differences were found between multiple administration groups for ovarian responses, high dosage of oFSH gave higher percent ovulation and CL per doe (72.4% ± 7.6vs. 58.8% ± 3.9and 16.9 ± 3.9vs. 11.6 ± 1.0, respectively) compared to low dosage. The anovulatory percentage of follicles was significantly (P<0.05) higher in Group S followed by Groups M, and M2. The structure (embryo plus oocyte) recovery rate per doe was significantly (P<0.05) higher in Group M2 followed by Group Ml and lowest in Group S (5.4 ± 2.4, 3.2 ± 1.2 and 0.5 ± 0.5, respectively). It is concluded that multiple injection at high dosage of oFSH in goat superovulation protocol gives better ovarian responses than multiple injection at low dosage as well as single injection of oFSH. However, more detailed research is needed to optimise the amount of gonadotrophin for superovulation of Malaysian crossbred goats.
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